https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41130 Wed 27 Jul 2022 10:35:19 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32990 SlMPK20 or its knockout using CRISPR/Cas9 significantly reduced or completely abolished pollen viability, respectively, with no effects on maternal fertility. Cell biology and gene expression analyses established that SlMPK20 exerts its role specifically at the uni-to-binucleate transition during microgametogenesis. This assertion is based on the findings that the transgenic pollen was largely arrested at the binucleate stage with the appearance of subcellular abnormality at the middle uninucleate microspore stage; and SlMPK20 mRNA and SlMPK20-GUS signals were localized in the tetrads, uninuclear microspores and binuclear pollen grains but not in microspore mother cells or mature pollen grains. Transcriptomic and proteomic analyses revealed that knockout of SlMPK20 significantly reduced the expression of a large number of genes controlling sugar and auxin metabolism and signaling in anthers. Finally, protein-protein interaction assays identified SlMYB32 as a putative target protein of SlMPK20. We conclude that SlMPK20 specifically regulates post-meiotic pollen development through modulating sugar and auxin metabolism and signaling.]]> Thu 28 Sep 2023 15:08:10 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52099 Thu 28 Sep 2023 14:50:07 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40991 Thu 21 Jul 2022 09:41:24 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:9443 Sat 24 Mar 2018 08:41:33 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:6985 Sat 24 Mar 2018 08:37:50 AEDT ]]>